Researchers from RIKEN Center for Sustainable Resource Science (CSRS) in Japan and collaborators from UniversitiSains Malaysia (USM) have successfully decoded the genome sequence for Hevea brasiliensis, the natural rubber tree native to Brazil. The study, published in Scientific Reports, shows a draft genome sequence that covers more than 93% of expressed genes and pinpoints regions specific to the biosynthesis of rubber.
When the plant becomes injured, natural rubber flows in latex ducts and protects it from insects. Natural rubber is an important resource for its many industrial applications because it has several useful properties that have not been reproducible in synthetic petroleum-based rubber. The reasons why some strains of rubber tree yield higher amounts of rubber than others are still unknown.
The team led by Minami Matsui at the RIKEN CSRS and Alexander Chong at USM set out to sequence and analyse the H. brasiliensis genome. Explains first author Nyok Sean Lau, “genomic information can reveal which genes contribute to the rubber tree’s capacity to produce high amounts of latex. This in turn will help us develop rubber trees with higher yields.”
After sequencing the rubber tree genome, researchers compared it to the genomes of other plants in the Euphobiaceae family, such as cassava and castor beans, and found that while they shared a large cluster of over 12,000 gene families, almost 2000 gene families were unique to the rubber tree.
The team looked for genes related to rubber itself and for resisting disease — two factors that affect rubber production. They found that the two most abundant proteins that make up rubber — Rubber Elongation Factor and Small Rubber Particle — are encoded on a large number of genes that are clustered together in a small region of the genome. While other tropical plants also express these proteins to some extent, being encoded more than eight times on different genes is unique to H. brasiliensis. Cap analysis gene expression (CAGE) — a method developed at RIKEN — revealed that expression of these proteins is tissue specific, with expression more than 100 times greater in latex than in leaves.
The team also found that compared to other members of the Euphobiaceae family, the rubber tree has many more genes related to disease resistance, and that these genes also formed clusters within the genome. “The high latex production found in the rubber tree,” theorizes Matsui, “may be caused by a coordinated expression of these genes combined with increased gene number due to gene duplication.”
“This finding will help improve latex production, a vital industrial crop in Malaysia,” notes Chong. “For USM, we hope to continue working with RIKEN and relevant industrial partners to utilize knowledge from genome for better production of rubber.”
Now that the genome in known, Matsui already has plans to use it for additional studies. “In addition to rubber quantity, we want to improve its quality by modifying rubber-synthesis pathways and developing synthetic biological production of rubber in microorganisms or green algae.”